Slides

Limitations of Current Dose-Response Data and Models: Information Needs for Microbiological Risk Assessors 
Margaret Coleman

1.     Dose-Response Relationships for Surrogate Pathogens 
2.     Dose-Response Relationships for Surrogate Pathogens 
3.     Dose-Response Relationships for Surrogate Pathogens 
4.     Dose-Response Relationships for Surrogate Pathogens 
5.     Dose-Response Relationships for Surrogate Pathogens 
6.     Observed Data and Fitted Curves for Surrogate Pathogens 
7.     Difficulties: Interpreting Data from Animal Models 
8.     System of Equations to Simulate the Number of Damaged Host Cells vs. Time 
9.     Theoretical Example for Three Alternative Model Forms 
10.   Theoretical Example for Three Alternative Model Forms 
11.   Linear vs. Non-Linear Behavior 
12.   Non-Threshold and Threshold Estimates of Risk 
13.   Disease Triangle 
14.   Family of Murine Dose-Response Relationships 
15.   Human Salmonellosis: "Average" for Healthy Adults 
16.   Family of Derived Human Salmonellosis Models 
17.   Salmonellosis Age Dependency 
18.   Family of Derived Human Salmonellosis Models 
19.   Pathogen Variability 
20.   Salmonella Strain Variability: Most Susceptible Population

Mechanisms of Pathogenesis of Salmonellae: Linking in vitro, Animal and Human Studies 
James Slauch, PhD

1.    Course of Infection 
2.    Peyer’s Patch 
3.    Salmonella Invading an Epithelial Cell 
4.    Type III Secretion System 
5.    Regulation of the Type III Secretion System 
6.    Phenotype Conferred by Mutations in Invasion System 
7.    Salmonella-induced Filaments 
8.    Attenuated Mutants as Vaccine Strains: Mice and Men

Correlating Host Resistance and Susceptibility with Biomarkers from in vitro, ex vitro and Animal Models 
H. Kirk Ziegler, PhD

1.   Listeria on a Macrophage [SEM] 
2.   Microbial Gene Expression, Virulence Factors and the Life-cycle of Listeria monocytogenes 
3.   T cells Binding to Infected Macrophage 
4.   Antigen Processing, Presentation and Recognition 
5.   Macrophage Response to Microbial Invasion 
6.   Science cover, 11 October 1996 
7.   Role of T cells and NK cells in Primary Resistance to Listeria 
8.   Model of Peritoneal Cell Dynamics During Infection 
9.   Model of Systemic Cytokine Dynamics During Infection 
10. Peritoneal T Cells From HKLM + IL-12 Immunized Mice Respond Specifically to Listerial Antigens in vitro
11. Immunization With HKLM + IL-12 Confers Protective Anti-Listerial Immunity 
12. Immunization with HKLM + IL-12 Confers Long-Lived Protective Immunity 
13. Control vs. Knock-out Mouse Strains 
14. Protective Immunity Conferred by Immunization with HKLM + IL-12 is Independent of MHC class I Expression 
15. Protective Immunity Conferred by Immunization with HKLM + IL-12 is Dependent on MHC class II Expression 
16. Protective Immunity Conferred by Immunization with HKLM + IL-12 is Dependent on IFN-g Expression 
17. Synthetic Peptides of LLO Elicit Strong Listerial Antigen-Specific Responses when Administered i.p. With IL-12 
18. Synthetic Peptides of LLO Elicit Strong Listerial Antigen-Specific Responses When Administered i.p. With IL-12 
19. Synthetic Peptides of LLO Elicit Protective Listeria-Specific Responses When Administered i.p. With IL-12 
20. Immune Response to Listeria